Miely Suarez The University of Arizona Graduate Research Assistant mielysuarez@arizona.edu 0009-0009-8506-8269 Elli Meinke The University of Arizona Undergraduate Research Assistant Viviana Romero The University of Arizona Undergraduate Research Assistant Zelieann Craig The University of Arizona Associate Professor zelieann@arizona.edu 0000-0002-9886-416X Miely Suarez The University of Arizona Graduate Research Assistant mielysuarez@arizona.edu 0009-0009-8506-8269 Phillip Carr Arizona State University Undergraduate Research Assistant Viviana Romero Undergraduate Research Assistant Elli Meinke Undergraduate Research Assistant ovary staining histology alcian blue hyaluronan hyaluronic acid AB stain follicles antrum formation This information is released under the Creative Commons license – Attribution-NonCommercial - CC BY-NC (https://creativecommons.org/licenses/by-nc/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User cannot use the material for commercial purposes. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you. Domain Eukaryota eukaryotes 2759 Kingdom Metazoa animals 33208 Phylum Chordata chordates 7711 Subphylum Craniata 89593 Superclass Sarcopterygii 8287 Class Mammalia mammals 40674 Superorder Euarchontoglires 314146 Order Rodentia rodents 9989 Suborder Myomorpha mice and others 1963758 Family Muridae 10066 Subfamily Murinae 39107 Genus Mus mice 10088 Subgenus Mus 862507 Species Mus musculus house mouse 10090 Zelieann Craig The University of Arizona Associate Professor zelieann@arizona.edu 0000-0002-9886-416X The slides were deparaffinized and hydrated in distilled water. Then, acetic acid was applied onto the section for 3 minutes. The excess solution was tipped off the slide and then Alcian Blue solution was applied to the slides and incubated for 25 minutes at 37°C. Slides were briefly rinsed with acetic acid for 30 seconds, rinsed in tap water and two changes of distilled water. Nuclear Fast Red solution was applied for 5 minutes then rinsed in tap water and two changes of distilled water. The slides were then dehydrated and coverslipped. Zelieann Craig The University of Arizona Associate Professor zelieann@arizona.edu 0000-0002-9886-416X Principal Investigator Approximately 7.5 million American women are unable to become pregnant or carry a baby to term (impaired fecundity). The ovary is considered a critical target organ in infertility cases based on the large number of women seeking infertility treatment who are unable to release a viable egg from their ovaries. Folliculogenesis is the process through which ovarian follicles, the functional units of the ovary, develop into a form capable of sustaining hormone production and ovulating a healthy egg for fertilization. Any environmental exposure that causes damage to the ovarian follicle has the potential to impair fecundity in women. Most of what is known about toxicity to ovarian follicles has been uncovered in laboratory rodents, but there is little available data on toxicant exposure and related mechanistic toxicology studies in the nonhuman primate, the closest laboratory animal model to humans. There is, therefore, a critical need to develop alternative approaches and tools that synergize the accessibility and versatility of laboratory rodents with the physiological relevance of the nonhuman primate to deliver high quality ovarian follicle toxicity data. To meet this need, this proposal will support the formation of a virtual research consortium with expertise in mouse and nonhuman primate ovarian biology, toxicology and computational modeling. The proposed consortium will generate lab-based data on the physiological and toxicant-induced behavior of ovarian follicles in both species, using phthalate as a model toxicant, to create computational models capable of effectively predicting ovarian follicle responses to chemical insults in nonhuman primates. The experimental measures captured in the in vitro and in vivo model will be specifically chosen to align with the needs of the modelers. To achieve this goal, the proposed consortium will complete three specific aims. Specifically, the ovarian biology/toxicology team will quantify the physiological and toxicant-induced behavior of computationally useful markers of in vivo and in vitro folliculogenesis in mice (Aim 1) and nonhuman primates (Aim 2), while the computational modeling team will construct in silico models that match the physiological behavior of folliculogenesis in each species and predict follicular responses to phthalate insults in both species. Our virtual consortium includes researchers from five institutions and will create a transdisciplinary group that includes both experimental and computational expertise. The tight coupling of these two domains will provide the needed communication pathways to develop translational models for IVIVE as well as cross-species extrapolations in reproductive toxicology. National Institute of Environmental Health Sciences R01ES034690-02 https://reporter.nih.gov/project-details/11018514 APOS001AB_SecondaryFollicle_5 APOS001AB_SecondaryFollicle_5.jpg jpg histology ZRC_MM_APOS001AB female unspecified 1 ovary unspecified 1I 5 microns Formalde-Fresh Solution Light microscopy stain Leica DMIL LED