Maria Sepulveda Purdue University marisep@purdue.edu 0000-0003-0473-5760 Maria Sepulveda Purdue University marisep@purdue.edu 0000-0003-0473-5760 Madison Van Slette Arizona State University Undergraduate Research Assistant Nancy Denslow 0000-0002-3946-3112 Collaborator Ting-Yu Chu Arizona State University Histology Slide Scanner Ethinyl estradiol (EE2) is a potent synthetic estrogen found in streams and rivers downstream from sewage waste water treatment plants. This study evaluated the reproductive effects of EE2 on adult fathead minnows. Fish were exposed to three concentrations of EE2 (2, 5 and 10 ng/L) for 30 days and effects on follicular development evaluated. pimephales promelas fathead minnow esthyl estradiol endocrine disruption ovary follicles This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you. 2006-01-25 Kingdom Metazoa metazoans 33208 Phylum Chordata chordates 7711 Subphylum Craniata 89593 Superclass Actinopterygii ray-finned fishes 7898 Class Actinopteri 186623 Subclass Neopterygii 41665 Infraclass Teleostei teleost fishes 32443 Superorder Cypriniphysae 186627 Order Cypriniformes carps and others 7952 Suborder Cyprinoidei 30727 Family Leuciscidae 2743726 Subfamily Pogonichthyinae 2743731 Genus Pimephales 51137 Species Pimephales promelas fathead minnow 90988 Maria Sepulveda Purdue University marisep@purdue.edu 0000-0003-0473-5760 Adult female fathead minnows were euthanized in buffered MS-222 (Ethyl 3-aminobenzoate methanesulfonate, Sigma, St. Louis, MO) (4 mg/mL). A sample of ovarian tissue was collected from either the right or the left ovary and fixed in 10% buffered formalin. Hematoxylin and eosin (H & E) sections were prepared following standard procedures and sections examined under a light microscope (10–40X) for staging and evaluation of any abnormalities. MS_PP_A06_10340_30_001a MS_PP_A06_10340_30_001a.ome.tif ome.tif Tank: 30; Weight (grams): 1.7; Length (mm): 51; K: 1.2815583749839805; Ovary weight (grams): 0.079; Publication: Li Z, Kroll KJ, Jensen KM, Villeneuve DL, Ankley GT, Brian JV, Sepúlveda MS, Orlando EF, Lazorchak JM, Kostich M, Armstrong B, Denslow ND, Watanabe KH. A computational model of the hypothalamic: pituitary: gonadal axis in female fathead minnows (Pimephales promelas) exposed to 17α-ethynylestradiol and 17β-trenbolone. BMC Syst Biol. 2011 May 5; 5:63. doi: 10.1186/1752-0509-5-63. PMID: 21545743; PMCID: PMC3118352. histology MS_PP_A06_10340_30 female adult 1 ovary right 001a Solvent Control Triethylene Glycol 5 microns Olympus CX33