Chad A Blanksma US EPA Great Lakes Toxicology and Ecology Division Histotechnologist

6201 Congdon Blvd. Duluth MN 55804 USA

blanksma.chad@epa.gov 0009-0001-8811-8083 This micrograph shows an ovary in a mid sagittal region from a female medaka aged 60 days post hatch. Populations of oogenic cell types that can be identified in this image include perinucleolar, cortical alveolar, and vitellogenic oocytes. Early and late development vitellogenic oocytes are observed in this region. This image was created by stitching several 10x images together. Scale bar = 500 microns. Medaka Adult Whole ovary Perinucleolar oocyte Cortical alveolar oocyte Vitellogenic oocyte Oogenesis Vitellogenesis This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you. 2016 Kingdom Animalia animals 202423 Subkingdom Bilateria triploblasts 914154 Infrakingdom Deuterostomia 914156 Phylum Chordata chordates 158852 Subphylum Vertebrata vertebrates 331030 Infraphylum Gnathostomata 914179 Superclass Actinopterygii ray-finned fishes 161061 Class Teleostei 161105 Superorder Acanthopterygii 166082 Order Beloniformes needlefishes 553128 Suborder Adrianichthyoidei 553156 Family Adrianichthyidae adrianichthyids 165623 Subfamily Oryziinae 645103 Genus Oryzias 165616 Species Oryzias latipes Japanese killifish 165622 Chad A Blanksma US EPA Great Lakes Toxicology and Ecology Division Histotechnologist blanksma.chad@epa.gov 0009-0001-8811-8083 Image collection device Diagnostic Instruments, Inc. SPOT Insight 12.3 Mp sCMOS/ 4K UHD Camera System Image collection software Diagnostic Instruments, Inc. SPOT Advanced Software Image analysis software Media Cybernetics Image-Pro Premier 9.3 Graphics Editing Software Corel Corporation CorelDraw Graphics Suite 2022 Chad A Blanksma US EPA Great Lakes Toxicology and Ecology Division Histotechnologist blanksma.chad@epa.gov 0009-0001-8811-8083 Contact TG_F0_Day_60_LSW1_76_1B_10x_Stitched_Cropped_Finished3 TG_F0_Day_60_LSW1_76_1B_10x_Stitched_Cropped_Finished3.tif tif histology USEPA_GLTED_OL_TransGen_BPA_EE2_02_LSW1_76 female 60 adult 1 ovary unspecified 1 5 microns Nikon Eclipse TE 300