Chad A Blanksma US EPA Great Lakes Toxicology and Ecology Division Histotechnologist

6201 Congdon Blvd. Duluth MN 55804 USA

blanksma.chad@epa.gov 0009-0001-8811-8083 This image created by stitching several 100x magnification images together shows a portion of ovary from an adult female fathead minnow. Oocytes at varying stages of development can be observed, along with post ovulatory follicles and oocyte atresia. Vitellogenic oocyte Perinucleolar oocyte Chromatin nucleolar oocyte Oocyte atresia Post-ovulatory follicle Adult Fathead minnow Germinal vesicle This information is released under the Creative Commons license - Attribution - CC BY (https://creativecommons.org/licenses/by/4.0/). The consumer of these data ("Data User" herein) is required to cite it appropriately in any publication that results from its use. The Data User should realize that these data may be actively used by others for ongoing research and that coordination may be necessary to prevent duplicate publication. The Data User is urged to contact the authors of these data if any questions about methodology or results occur. Where appropriate, the Data User is encouraged to consider collaboration or co-authorship with the authors. The Data User should realize that misinterpretation of data may occur if used out of context of the original study. While substantial efforts are made to ensure the accuracy of data and associated documentation, complete accuracy of data sets cannot be guaranteed. All data are made available "as is." The Data User should be aware, however, that data are updated periodically and it is the responsibility of the Data User to check for new versions of the data. The data authors and the repository where these data were obtained shall not be liable for damages resulting from any use or misinterpretation of the data. Thank you. 2015 Kingdom Animalia animals 202423 Subkingdom Bilateria triploblasts 914154 Infrakingdom Deuterostomia 914156 Phylum Chordata chordates 158852 Subphylum Vertebrata vertebrates 331030 Infraphylum Gnathostomata 914179 Superclass Actinopterygii ray-finned fishes 161061 Class Teleostei 161105 Superorder Ostariophysi 162845 Order Cypriniformes minnows 162846 Superfamily Cyprinoidea 638796 Family Leuciscidae 1198935 Subfamily Pogonichthyinae 1198937 Genus Pimephales bluntnose minnows 163515 Species Pimephales promelas Fathead Minnow 163517 Chad A Blanksma US EPA Great Lakes Toxicology and Ecology Division Histotechnologist

Duluth MN 55804 USA

blanksma.chad@epa.gov 0009-0001-8811-8083 Image collection device Diagnostic Instruments, Inc. SPOT Insight 4 Mp CCD Scientific Color Digital Camera Image collection software Diagnostic Instruments, Inc. SPOT Advanced Software Image analysis software Media Cybernetics Image-Pro Premier Graphics Editing Software Corel Corporation Corel Photo-Paint Chad A Blanksma US EPA Great Lakes Toxicology and Ecology Division Histotechnologist blanksma.chad@epa.gov 0009-0001-8811-8083 Contact C4A1_Corel_photopaint_stitched_scalebar_10x C4A1_Corel_photopaint_stitched_scalebar_10x.tif tif histology USEPA_GLTED_PP_Indom21d_C4A1 female adult 1 ovary unspecified 1A 4 microns Zeiss Axiovert 35